Rapid Real-Time PCR Detection of HPdel Directly from Diluted Blood Samples

Tion of Plasma Volume Changes during Marathon Rapid Real-time Pcr Detection of Hp Del Directly from Diluted Blood Samples Letters to the Editor

Anhaptoglobinemic patients have been reported to experience severe anaphylactic reactions to transfusions due to the production of antihaptoglobin (anti-HP) antibodies (1, 2 ). Anhaptoglobinemia in patients homozygous for HP , which is a deletion of an approximately 28-kb segment of chromosome 16 extending from the promoter region of the HP (haptoglobin) gene to exon 5 of HPR (haptoglobin-relat...

A rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin

The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...

A rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin

The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...

Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction

Rapid detection of Klebsiella pneumoniae carbapenemase genes in enterobacteriaceae directly from blood culture bottles by real-time PCR.

Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae are endemic in New York City hospitals and have been associated with serious infections globally. A real-time polymerase chain reaction (RT-PCR) assay was developed to detect carbapenem resistance attributable to KPC from blood culture bottles positive for gram-negative bacilli. Culture confirmation of carbapenemase producti...